Journal: Parasites & Vectors

Article Title: Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes

doi: 10.1186/s13071-023-06045-z

Figure Lengend Snippet: Development of genotype-specific polymerase chain reaction (PCR) assays for Theileria equi . a Primer design for T. equi genotype-specific PCR assays. 18S ribosomal RNA (18S rRNA) gene sequences of T. equi were aligned, and a set of forward and reverse primers specific to each genotype was designed. The specific binding regions (highlighted in gray) of the forward and reverse primers on the 18S rRNA sequences representing genotypes A (JX177673), B (EU642507), C (JQ390047), D (AB515312), and E (KF559357) are shown. The dashes represent gaps. b Nucleotide polymorphisms among the 18S rRNA sequences targeted by the genotype-specific PCR assays. The 18S rRNA sequences targeted by the PCR assays specific to T. equi genotypes A, B, C, D, and E were trimmed and aligned, and the resultant 687-base pair (bp) alignment was analyzed for nucleotide polymorphisms. The dots and dashes represent identical nucleotides and gaps, respectively, to those in the genotype A sequence. Conserved nucleotide polymorphisms in each genotype are shown

Article Snippet: Fig. 1 Development of genotype-specific polymerase chain reaction (PCR) assays for Theileria equi . a Primer design for T. equi genotype-specific PCR assays.

Techniques: Polymerase Chain Reaction, Binding Assay, Sequencing

Journal: Parasites & Vectors

Article Title: Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes

doi: 10.1186/s13071-023-06045-z

Figure Lengend Snippet: Primers used in Theileria equi genotype-specific polymerase chain reaction assays

Article Snippet: Fig. 1 Development of genotype-specific polymerase chain reaction (PCR) assays for Theileria equi . a Primer design for T. equi genotype-specific PCR assays.

Techniques: Polymerase Chain Reaction, Amplification

Journal: Parasites & Vectors

Article Title: Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes

doi: 10.1186/s13071-023-06045-z

Figure Lengend Snippet: Specificity of the genotype-specific PCR assays for Theileria equi . Each genotype-specific PCR assay was evaluated for its ability to differentially detect the target genotype from the remaining genotypes and DNA of Babesia caballi and noninfected horse blood. The genotype-specific PCR assays were highly specific in detecting their target genotypes. A – E Plasmid DNA with inserts of 18S rRNA from T. equi genotypes A–E, respectively; BC DNA of B. caballi ; H DNA of noninfected horse blood; N negative control (no template); M 100-bp DNA ladder; for other abbreviations, see Fig.

Article Snippet: Fig. 1 Development of genotype-specific polymerase chain reaction (PCR) assays for Theileria equi . a Primer design for T. equi genotype-specific PCR assays.

Techniques: Plasmid Preparation, Negative Control

Journal: Parasites & Vectors

Article Title: Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes

doi: 10.1186/s13071-023-06045-z

Figure Lengend Snippet: Phylogenetic analysis of Theileria equi 18S rRNA. The 18S rRNA sequences amplified in the genotype-specific PCR assays, together with reference sequences representing each genotype, were used to construct a maximum-likelihood phylogeny with 1000 bootstrap replicates. The newly determined sequences from donkeys from Sri Lanka (in red) and horses from Paraguay (in blue) from the amplicons from each genotype-specific PCR assay clustered in the corresponding clades. For abbreviations, see Fig.

Article Snippet: Fig. 1 Development of genotype-specific polymerase chain reaction (PCR) assays for Theileria equi . a Primer design for T. equi genotype-specific PCR assays.

Techniques: Amplification, Construct